MEIOTHERMUS RUBER
Principle Project Collaborators:
- Dr. Lori Scott, Augustana College, Rock Island, IL
- Ms. Angela Ghrist, Scott Community College, Bettendorf, IA
- Dr. Matthew Halfhill, St. Ambrose University, Davenport, IA
- Dr. Todd Linscott, BlackHawk College, Moline, IL
Additional Collaborators
- Dr. Kristin Douglas, Augustana College
- Dr. Jason Singer, Augustana College
- Dr. Wegman-Geedey, Augustana College
Important Links
Number of ORFs with partial annotations as of 12-02-09 ....................23
Important Publications and Documents
A student version of the instructions for Modules 1-5 of the IMG-ACT annotation for Meithermus ruber is available for network members only; request a copy by emailing Lori Scott. Instructions for the remaining modules will be available soon.
Latest research findings
1. Genomic DNA from Meiothermus ruber was isolated from a 1-day overnight culture using the Wizard SV DNA Purification System. We followed the instructions provided by McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes.
Figure 1: Isolation of gDNA from M. ruber and E. coli and the Subsequent Amplification of the 16S rRNA gene
- Average concentration of M. ruber gDNA = 6.4ug in 200ul elution buffer
- Average concentration of E. coli gDNA = 2.5ug in 200ul elution buffer
This project was performed by the BI375/Molecular Genetics students from Winter 2009-10.
2. The BI375 research team is currently annotating 20 M. ruber genes and cloning a subset of these genes.