Links |
MEIOTHERMUS RUBER GENOME ANNOTATION PROJECT Principle Project Collaborators:
Additional Collaborators
|
|
Important News.
Important Publications and Documents
|
Annotation up-date Number of ORFs with completed annotations as of 07-01-10 ...............35 Number of ORFs with partial annotations as of 07-01-10 ........................10 |
|
|
Latest research findings 1. Genomic DNA from Meiothermus ruber was isolated from a 1-day overnight culture using the Wizard SV DNA Purification System. We followed the instructions provided by McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes. Figure 1: Isolation of gDNA from M. ruber and E. coli and the Subsequent Amplification of the 16S rRNA gene. This project was performed by the BI375/Molecular Genetics students from Winter 2009-10
2. The BI375 research team is currently annotating 30 M. ruber genes and cloning a subset of these genes. The outcome of this project will be presented at the ISAS meeting in April, 2010. 3. Angela Ghrist (EICCD/Scott Community College) and Nicole Oldfather confirmed through restriction enzyme analysis that we had successfully amplified the 16SrRNA gene(s) using universal primers purchased from IDT (Coralville, IA). They are working to clone the beta-galactosidase gene into a suitable vector. 4. A poster was presented at the 2010 Illinois State Academy of Sciences 102nd annual meeting from the collaborative work of the 2009-10 BI375 students, and A. Ghrist and N. Oldfather from Scott Community College (Click Here) |
||
Last updated: 08/01/2010 11:04:04 AM