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Important News.
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MEIOTHERMUS RUBER GENOME ANNOTATION PROJECT Principle Project Collaborators:
Additional Collaborators
Contact information - meiothermus@gmail.com Coming soon: Meiothermus Facebook page
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Annotation up-date
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Latest research findings 1. Genomic DNA from Meiothermus ruber was isolated from a 1-day overnight culture using the Wizard SV DNA Purification System. We followed the instructions provided by McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes.
2. The BI375 2009-10 research team annotated 30 M. ruber genes and cloned three of these genes into a pUC18 plasmid. 3. Angela Ghrist (EICCD/Scott Community College) and Nicole Oldfather confirmed through restriction enzyme analysis that we had successfully amplified the 16SrRNA gene(s) using universal primers purchased from IDT (Coralville, IA). They are working to clone the beta-galactosidase gene into a suitable vector. 4. A poster was presented at the 2010 Illinois State Academy of Sciences 102nd annual meeting from the collaborative work of the 2009-10 BI375 students, and A. Ghrist and N. Oldfather from Scott Community College (Click Here) 5. Dr. Scott appears to have demonstrated complementation between an E. coli ArgH- auxotroph and a Meiothermus ruber ArgH+ gene cloned into the pKt-1 plasmid. The technique is one developed by GENI. M. ruber has two ArgH genes, identified as ArgH1 and ArgH2. We attempted to complement both genes from M. ruber, but only one of two genes successfully complemented. The next step is to sequence the two clones to confirm/refute if they are ArgH1 and ArgH2. |
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| http://sigen.org/index.php/sigen/article/viewArticle/sigs.1032748 | ||
Last updated: 01/24/2011 04:21:19 PM